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Tag Archive - Genome Sequencing

NGS-Different-Sanger-Sequencing

How is NGS Different from Sanger Sequencing?

Introduction Sequencing DNA involves biochemical processes to ascertain the order (sequence) of nucleotides of DNA strands. Maxam-Gilbert and Sanger methods were developed over 40 years ago, but the Sanger method soon dominated the DNA sequencing arena. With this method, DNA is repeatedly copied resulting in DNA fragments of varying lengths. The ends of each fragment are labeled with a chain terminator, and this allows the determination of the DNA sequence.…

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race-and-predisposition

Race and Predisposition to Disease

Introduction Interdisciplinary efforts over several decades have provided intriguing information regarding the factors that contribute to racial differences in health status and outcomes. Even with this information on hand, challenges remain regarding improved characterization of these various biological and environmental factors. Advances in genomic studies have provided some of the valuable tools and data needed to move forward in the direction of defining the biologic factors underlying racial differences in…

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NGS-life-expectancy

Next Generation Sequencing Could Dramatically Impact Life Expectancy

Introduction A Centers for Disease Control website provides a definition for life expectancy as “the average number of years that a hypothetical group of infants would live at each attained age if the group was subject, throughout its lifetime, to the age-specific death rates prevailing for the actual population in a given year” (1). It is essentially the time a person or other organism is expected to live based on…

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Chromatin Immunoprecipitation-sequencing-macrogen

Chromatin Immunoprecipitation-sequencing (ChIP-seq)

Chromatin Immunoprecipitation assays have the ability to capture an image of specific protein to DNA interactions that are occurring in a system and process these interactions using quantitative polymerase chain reaction (qPCR), ChIP-chip, or next generation sequencing (NGS). When paired with next generation sequencing specifically, ChIP-seq is a powerful way to identify genome-wide DNA binding sites for transcription factors and other proteins. When performing ChIP-seq, chromatin is separated from cells…

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Pancreatic Cancer

Role of Next-Generation Sequencing in Pancreatic Cancer Management

Introduction Pancreatic cancer (PC) is considered one of the most fatal cancers in the U.S. and other developed countries. The best outcomes and survival rates after treatment are only possible with early detection and treatment before the onset of symptoms. There is a great need to uncover ways to detect PC early to achieve better clinical outcomes. To date, there are no reliable means for the early, pre-symptom detection of…

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genome sequencing

The Race for the $1,000 Genome: The Increasing Speed and Accuracy of DNA Sequencing

Science fiction fans remember the Star Trek tricorders that rapidly rendered a diagnosis, or the database that could provide the entire past and present information (even the molecular details) of a scanned planet. The state of science, as we live it today, reflects the path to bring many of these concepts into fruition. Although discovering the details of the massive human genome seems a dream or a science fiction-quality endeavor,…

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Using Exome Sequencing to Aid in Diagnosing Ataxias

Clinical exome sequencing has proven to be an extremely valuable tool in the world of diagnostics as explained by Christopher M. Gomez, MD, PhD and Soma Das, PhD. While the volume of genetic information continues to grow, one of its many applications can be applied to conditions such as ataxia. Many studies have been performed using exome sequencing and while the determining factors were not spelled out directly, the authors…

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Dr. Michael Snyder: Interpretation of Whole Genome Sequencing in Clinics

Whole genome sequencing (WGS) is a service that has held a lot of promise for the clinical and personalized medicine world. Having treatments and regiments that were specific to your own unique needs is something that many are striving to achieve based off of WGS findings. Dr. Snyder and his colleagues, such as Frederick E. Dewey, MD, Jason D. Merker, MD, PhD, and Atul J. Butte, MD, PhD, et. al. held a study with 12 participants…

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Ebola Outbreak Leaves Behind Genome Sequences

On August 28, 2014, researchers from Harvard University and the Broad Institute released a report that sequences and analyzes the genomes from the recent outbreak of the Ebola virus in Sierra Leone.   Stephen Gire and Pardis Sabeti of Harvard and the Broad Institute led the collaborative effort of more than 50 co-authors. The research is based on examples of the virus from 78 people who were diagnosed with Ebola…

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Human Whole Genome Sequencing

Human Whole Genome Sequencing Whole genome sequencing or full genome sequencing is a field of study that has proven to be quite critical to the average person. The number of discoveries found through sequencing is rapidly growing by the day.   Of the many applications for genome sequencing, one of the largest is for research. This might apply to finding links between certain genes and the susceptibility to having certain…

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