Our Blog

Tag Archive - Next Generation Sequencing (NGS)

NGS-Different-Sanger-Sequencing

How is NGS Different from Sanger Sequencing?

Introduction Sequencing DNA involves biochemical processes to ascertain the order (sequence) of nucleotides of DNA strands. Maxam-Gilbert and Sanger methods were developed over 40 years ago, but the Sanger method soon dominated the DNA sequencing arena. With this method, DNA is repeatedly copied resulting in DNA fragments of varying lengths. The ends of each fragment are labeled with a chain terminator, and this allows the determination of the DNA sequence.…

Read more Leave a comment

Chromatin Immunoprecipitation-sequencing-macrogen

Chromatin Immunoprecipitation-sequencing (ChIP-seq)

Chromatin Immunoprecipitation assays have the ability to capture an image of specific protein to DNA interactions that are occurring in a system and process these interactions using quantitative polymerase chain reaction (qPCR), ChIP-chip, or next generation sequencing (NGS). When paired with next generation sequencing specifically, ChIP-seq is a powerful way to identify genome-wide DNA binding sites for transcription factors and other proteins. When performing ChIP-seq, chromatin is separated from cells…

Read more Leave a comment

recombinant-vaccines-macrogen

Sequencing Data to Develop Recombinant Vaccines

Introduction Eradication and prevention of infectious diseases are achieved most often via vaccination. The common or traditional vaccines are made using the actual pathogen material, such as modified live or killed (inactivated) viruses. The use of these types of vaccines is sometimes associated with serious side effects. For some infectious agents, it is often difficult to develop vaccines using this approach, and there are limitations on the effectiveness of these…

Read more One Comment

drug resistant bacteria

Discovering Drug Resistance Using DNA Sequencing

What is Drug Resistance? The World Health Organization (WHO) defines drug resistance (also called antimicrobial resistance) as the change that occurs in microorganisms that renders medications ineffective. There are other types of drug resistance, such as cancer cell resistance to chemotherapeutic agents. This discussion, however, will focus on antimicrobial drug resistance. As antimicrobial drugs become less effective, it is more difficult to combat infections that were previously manageable. Developing means…

Read more Leave a comment

Macrogen and Silicon Biosystems Menarini to Co-Develop CLIA-Certified Genomic Cancer Assays

Collaboration Aims to Integrate Digital Sorting with Next Generation Sequencing (NGS) of Formalin-Fixed Paraffin-Embedded Biopsies and Fine-Needle Aspiration Samples SAN DIEGO and SEOUL, South Korea, Feb. 12, 2016 /PRNewswire/ — Silicon Biosys­tems Menarini and Macrogen, Inc. today announced they will form a partnership to provide clinical assays and innovative procedures for precision medicine in cancer. The goal of the collaboration is to combine Silicon Biosystems’ DEPArray™ digital-sorting technology with Macrogen’s…

Read more Leave a comment

Tablet being used for personalized medicine

DNA Sequencing: The Backbone of Personalized Medicine

Concept of Personalized Medical Care As a society, we have been accustomed to the concept of a “one-size-fits-all” approach to healthcare. However, this has not been ideal for many people and limits the success of medical management. There are instances where there is some level of tailoring to the individual, but not to the extent needed to address the numerous nuisances that can affect the optimal results of a person’s…

Read more Leave a comment

NGS DNA Sequencing

Next-Generation Sequencing Revolutionizes Drug Discovery and Development

Next-Generation Sequencing (NGS) Overview Next-generation sequencing is a massive parallel sequencing technology. Genomic DNA is extracted, fragmented, and linked to adapters and primers for the amplification reaction (PCR) to generate a library. DNA fragments in the library are simultaneously sequenced. This provides a massive set of sequence data in a matter of days. The data obtained is processed with bioinformatics software and interpreted. In addition to rapidly obtaining sequence data…

Read more Leave a comment

DNA sequencing

Role of Next-Generation Sequencing in Cancer Diagnostics

The significant global burden of cancer has led to increased efforts to determine prevention strategies, early detection, and successful management of patients diagnosed with cancer. The latest data provided by the World Health Organization reveal cancer has accounted for 8.2 million deaths as of 2012¹. Early detection and successful treatment are considered important factors in reducing cancer incidence and mortality. Accurate and early diagnosis is necessary for an appropriate treatment…

Read more Leave a comment